Anti-Cdc27 Antibody (AF3.1) is a mouse monoclonal IgG2b κ Cdc27 antibody, cited in 66 publications, provided at 200 µg/ml
raised against amino acids 814-823 of Cdc27 of human origin
Cdc27 Antibody (AF3.1) is recommended for detection of Cdc27 of mouse, rat, human and Xenopus origin by WB, IP, IF and IHC(P); also reactive with additional species, including and equine and canine
Anti-Cdc27 Antibody (AF3.1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdc27 (AF3.1): sc-9972.
m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc27 Antibody (AF3.1) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdc27 Antibody (AF3.1) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Cdc27 Antibody (AF3.1) is a high quality monoclonal Cdc27 antibody (also designated H-NUC antibody, Anaphase-promoting complex subunit 3 antibody or ANAPC3 antibody) suitable for the detection of the Cdc27 protein of mouse, rat, human and Xenopus laevis origin. Cdc27 Antibody (AF3.1) is available as both the non-conjugated anti-Cdc27 antibody form, as well as multiple conjugated forms of anti-Cdc27 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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