Anti-Cdc27 Antibody (AF3.1) recommended for detection of Cdc27 of mouse, rat, human and Xenopus origin by WB, IP, IF and IHC(P)

Anti-Cdc27 Antibody (AF3.1): sc-9972

Anti-Cdc27 Antibody (AF3.1) is rated 5.0 out of 5 by 11.
  • y_2021, m_10, d_22, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_en_US, sid_9972, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 131ms
  • REVIEWS, PRODUCT
loading
5
11
4
0
3
0
2
0
1
0

Datasheets

    • Anti-Cdc27 Antibody (AF3.1) is a mouse monoclonal IgG2b κ Cdc27 antibody, cited in 66 publications, provided at 200 µg/ml
    • raised against amino acids 814-823 of Cdc27 of human origin
    • Cdc27 Antibody (AF3.1) is recommended for detection of Cdc27 of mouse, rat, human and Xenopus origin by WB, IP, IF and IHC(P); also reactive with additional species, including and equine and canine
    • Anti-Cdc27 Antibody (AF3.1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdc27 (AF3.1): sc-9972.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc27 Antibody (AF3.1) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdc27 Antibody (AF3.1) (see ordering information below).
loading

Can you recommend a positive control to use with Cdc27 (AF3.1): sc-9972 for Western blot?

Asked by: jenni
We recommend K-562 (sc-2203), RAW 264.7 (sc-2211) or HeLa (sc-2200) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of Rab 7 in these cell lines.
Answered by: Technical Support 13
Date published: 2017-01-20

How can Cdc27 (AF3.1): sc-9972 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. Cdc27 (AF3.1): sc-9972 monoclonal antibody is currently available conjugated to phycoerythrin (sc-9972 PE), fluorescein (sc-9972 FITC), Alexa Fluor® 488 (sc-9972 AF488) or Alexa Fluor® 647 (sc-9972 AF647)
Answered by: Technical Support 2
Date published: 2017-01-16
  • y_2021, m_10, d_22, h_18CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_9972, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 114ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from good product It is a good antibody and works well for western blot.
Date published: 2019-10-28
Rated 5 out of 5 by from good product It is a good antibody and works well for western blot.
Date published: 2018-08-22
Rated 5 out of 5 by from Outstanding Very strong western blot data; also superb IF and IHC staining
Date published: 2017-01-25
Rated 5 out of 5 by from Great IF staining We used this antibody for IF staining and got great images and nice intensity form HeLa cells nucleus
Date published: 2017-01-18
Rated 5 out of 5 by from Works well in IP & WB Works well in IP & WB in detecting endogenous CDC27 in HeLa cells. The rest of the APC/C co-IPs with CDC27.
Date published: 2016-12-06
Rated 5 out of 5 by from This antibody worked well in out blot in This antibody worked well in out blot in HeLa cells.
Date published: 2015-06-05
Rated 5 out of 5 by from Good WB antibody in rat cerebellar granule neurons Good WB antibody in rat cerebellar granule neurons. -SCBT Publication Review
Date published: 2015-04-27
Rated 5 out of 5 by from Worked well in WB in human transfected HeLa cells Worked well in WB in human transfected HeLa cells. -SCBT Publication Review
Date published: 2015-04-15
  • y_2021, m_10, d_22, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_en_US, sid_9972, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT