Anti-Cdc25B Antibody (DCS-164) recommended for detection of of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Cdc25B Antibody (DCS-164): sc-65504

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Anti-Cdc25B Antibody (DCS-164) is rated 2.0 out of 5 by 1.
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Datasheets

    • Cdc25B Antibody (DCS-164) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
    • raised against full length Cdc25B of human origin
    • recommended for detection of of mouse, rat and human origin by WB, IP, IF and ELISA
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Cdc25B Antibody (DCS-164) for WB applications. These reagents are now offered in bundles with Cdc25B Antibody (DCS-164) (see ordering information below).
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I am using Cdc25B (DCS-164): sc-65504 for immunofluorescence, what dilution of this antibody should I use?

Asked by: TinTin
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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