Anti-Cdc25A Antibody (F-6) is a mouse monoclonal IgG1 κ Cdc25A antibody, cited in 233 publications, provided at 200 µg/ml
epitope mapping at the C-terminus of Cdc25A of mouse origin
Anti-Cdc25A Antibody (F-6) is recommended for detection of Cdc25A of mouse, rat and human origin by WB, IP and IF
Anti-Cdc25A Antibody (F-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdc25A (F-6): sc-7389.
m-IgG1 BP-HRP is the preferred secondary detection reagent for Cdc25A Antibody (F-6) for WB applications. This reagent is now offered in a bundle with Cdc25A Antibody (F-6) (see ordering information below).
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
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Labware ships FedEx Ground free of charge to the contiguous US
Cdc25A Antibody (F-6) is a high quality monoclonal Cdc25A antibody (also designated M-phase inducer phosphatase 1 antibody) suitable for the detection of the Cdc25A protein of mouse, rat and human origin. Cdc25A Antibody (F-6) is available as both the non-conjugated anti-Cdc25A antibody form, as well as multiple conjugated forms of anti-Cdc25A antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The Cdc2/cyclin B enzyme, involved in regulation of mitosis in eukaryotic cells, is subject to multiple levels of control. Among these, the regulation of the catalytic subunit by Tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B complex, while Tyrosine dephosphorylation, which occurs at the onset of mitosis, directly activates the pre-MPH complex. The Cdc25 gene serves as a rate-limiting mitotic activator, apparently due to its action as the Cdc2 Tyrosine phosphatase. In the absence of Cdc25, Cdc2 accumulates in a Tyrosine phosphorylated state. In addition, Cdc25 proteins from a variety of species have been shown to share a low degree of sequence similarity with other Tyrosine phosphatases. The Cdc25 gene family consists of at least three members that share approximately 40% identity in their most conserved carboxy-terminal sequences.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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