Anti-Cdc25A Antibody (5H51) recommended for detection of Cdc25A of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-Cdc25A Antibody (5H51): sc-70824

Anti-Cdc25A Antibody (5H51) is rated 3.0 out of 5 by 1.
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Datasheets

    • Cdc25A Antibody (5H51) is a mouse monoclonal IgG2a κ, cited in 4 publications, provided at 200 µg/ml
    • raised against purified recombinant Cdc25A
    • recommended for detection of Cdc25A of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See Cdc25A (F-6): sc-7389 for Cdc25A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc25A Antibody (5H51) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdc25A Antibody (5H51) (see ordering information below).
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Can Cdc25A (5H51): sc-70824 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, Cdc25A (5H51): sc-70824 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 3 out of 5 by from Worked well with transfected 293 whole cell Worked well with transfected 293 whole cell lysates by WB. - SCBT QC
Date published: 2013-06-17
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