Anti-Cdc25A Antibody (3H2016) recommended for detection of Cdc25A of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-Cdc25A Antibody (3H2016): sc-70823

Anti-Cdc25A Antibody (3H2016) is rated 3.7 out of 5 by 3.
  • y_2021, m_11, d_29, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_en_US, sid_70823, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 274ms
  • REVIEWS, PRODUCT
loading
5
0
4
2
3
1
2
0
1
0

Datasheets

    • Cdc25A Antibody (3H2016) is a mouse monoclonal IgG2a κ, cited in 5 publications, provided at 200 µg/ml
    • raised against purified recombinant Cdc25A
    • recommended for detection of Cdc25A of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See Cdc25A (F-6): sc-7389 for Cdc25A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc25A Antibody (3H2016) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdc25A Antibody (3H2016) (see ordering information below).
loading

Can Cdc25A (3H2016): sc-70823 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, Cdc25A (3H2016): sc-70823 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
  • y_2021, m_11, d_29, h_18CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_70823, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 103ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Works well with human Cdc25A transfected Works well with human Cdc25A transfected 293 whole cell lysates. -SCBT QC
Date published: 2015-03-03
Rated 4 out of 5 by from Published data in a cohort of 313 patients Published data in a cohort of 313 patients using this antibody for Western blot analysis. -SCBT Publication Review
Date published: 2015-01-11
Rated 3 out of 5 by from Strong band with some background for WB in Strong band with some background for WB in HeLa (A) and K-562 (B) whole cell lysates. -SCBT QC
Date published: 2014-11-13
  • y_2021, m_11, d_29, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_en_US, sid_70823, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 160ms
  • REVIEWS, PRODUCT