Anti-Cdc2 p34 Antibody (B-5) recommended for detection of Cdc2 p34 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Cdc2 p34 Antibody (B-5): sc-137035

Anti-Cdc2 p34 Antibody (B-5) is rated 4.8 out of 5 by 5.
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Datasheets

    • Cdc2 p34 Antibody (B-5) is a mouse monoclonal IgG1 κ, cited in 9 publications, provided at 200 µg/ml
    • raised against amino acids 1-297 representing full length Cdc2 p34 of human origin
    • recommended for detection of Cdc2 p34 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See Cdc2 p34 (17): sc-54 for Cdc2 p34 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for Cdc2 p34 Antibody (B-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with Cdc2 p34 Antibody (B-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Can Cdc2 p34 (B-5): sc-137035 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, Cdc2 p34 (B-5): sc-137035 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Works well for WB with human ovarian surface Works well for WB with human ovarian surface epithelial cells and cancer cell lines. -SCBT Publication Review
Date published: 2015-03-02
Rated 5 out of 5 by from Clean WB blot with single strong band in Clean WB blot with single strong band in human U373MG. -SCBT Publication Review
Date published: 2015-03-01
Rated 5 out of 5 by from Very good nuclear staining in HeLa cells Very good nuclear staining in HeLa cells. -SCBT QC
Date published: 2015-01-27
Rated 5 out of 5 by from Single strong band at correct mw in HeLa Single strong band at correct mw in HeLa. -SCBT QC
Date published: 2015-01-10
Rated 4 out of 5 by from Good specificity and signal strength with Good specificity and signal strength with little background for WB in K-562 , A-431 and HL-60 whole cell lysates -SCBT QC
Date published: 2014-03-29
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