Anti-Cdc2 p34 Antibody (17) recommended for detection of Cdc2 p34 of mouse, rat, human and Xenopus origin by WB, IP, IF and IHC(P)

Anti-Cdc2 p34 Antibody (17): sc-54

Anti-Cdc2 p34 Antibody (17) is rated 4.7 out of 5 by 43.
  • y_2021, m_10, d_24, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_43
  • loc_en_US, sid_54, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 7ms
  • REVIEWS, PRODUCT
loading
5
34
4
6
3
1
2
1
1
1

Datasheets

    • Anti-Cdc2 p34 Antibody (17) is a mouse monoclonal IgG2a κ Cdc2 p34 antibody, cited in 685 publications, provided at 200 µg/ml
    • raised against amino acids 224-230, mapping within a central region of Cdc2 p34 of human origin
    • Cdc2 p34 Antibody (17) is recommended for detection of Cdc2 p34 of mouse, rat, human and Xenopus origin by WB, IP, IF and IHC(P)
    • Anti-Cdc2 p34 Antibody (17) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to TRITC for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdc2 p34 (17): sc-54.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc2 p34 Antibody (17) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdc2 p34 Antibody (17) (see ordering information below).
loading

Any information if this antibody will react bovine protein? Thank you.

Asked by: clvleal
Thank you for your question. We are aware of one publication citing use of this antibody in Madin-Darby bovine kidney (MDBK) cells. Please refer to this reference on NCBI's PubMed, PMID: 9733854.
Answered by: Technical Support
Date published: 2017-08-02

Can this antibody be used to detect Cdc2 in yeast?

Asked by: AbPolly
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions about reactivity with unlisted species.
Answered by: Technical Service
Date published: 2016-12-22

Which version of this antibody should be used for IP, sc-54 or sc-54 AC?

Asked by: AbPolly
Thank you for your inquiry. If the only application you intend to use this antibody for is IP, then the agarose conjugated version sc-54 AC is a great choice. If you need to use the antibody for multiple applications such as IP and Western Blot, then the unconjugated version sc-54 is best.
Answered by: Technical Service
Date published: 2016-12-22
  • y_2021, m_10, d_24, h_19CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_en_US, sid_54, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 7ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Good enough The antibody works fine in IHC on FFPE. The best dilution is 1:400 using as antigen retrival EDTA buffer pH 8.0 in water bath at 98°C. The sample in the photos are bioptical sample from bladder carcinoma. The staining is heterogenous between cancer cells, mainly in the cytoplasm but occasionally found in nuclei. Magnification 20x and 40x.
Date published: 2021-04-27
Rated 4 out of 5 by from molto buono Questa anticorpo è stato acquistato il 9 Febbraio; risultato molto buono.
Date published: 2021-03-18
Rated 5 out of 5 by from Great immunoblotting antibodies! Although this antibody was raised against human Cdc2, it immunoblots Cdc2 in Xenopus egg extracts very well with low background staining. Although there is one cross-reactive band above 60 kDa, it is far enough from the Cdc2 signal to obtain clear cut results. /Users/jxkuang/Desktop/sc-54.png
Date published: 2021-01-27
Rated 4 out of 5 by from 对应条带清晰,特异性较高 本抗体,我们试验中用于大鼠组织标本蛋白检测,条带较为清晰,对应分子量匹配,特异性较高,后期如果需要会继续购入。
Date published: 2018-11-14
Rated 5 out of 5 by from Great product!! It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 4 out of 5 by from 效果还不错 申请的小样,发的条带在34KDa处左右,评论区有人说70KDa处也有这个带,本人未亲自尝试。总之,效果还不错。
Date published: 2018-04-08
Rated 2 out of 5 by from 不理想 两个月前申请的试用小样,初次使用条带位置不正确,后来位置一直不在34kd左右,给实验带来一点麻烦!!
Date published: 2017-12-19
Rated 1 out of 5 by from 有点不理想 使用的是小样产品,,说明书上条带位于34kd左右,,但是我做出来的条带接近于70kd,,这是怎么回事呢!
Date published: 2017-12-12
  • y_2021, m_10, d_24, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_43
  • loc_en_US, sid_54, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 16ms
  • REVIEWS, PRODUCT