CD83 Antibody (F-5)recommended for detection of CD83 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA

CD83 Antibody (F-5): sc-55535

CD83 Antibody (F-5) is rated 4.7 out of 5 by 3.
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Datasheets

    • CD83 Antibody (F-5) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 7-205 mapping at the C-terminus of CD83 of human origin
    • CD83 Antibody (F-5) is recommended for detection of CD83 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA
    • CD83 Antibody (F-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See CD83 (D-3): sc-55536 for CD83 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD83 (F-5): sc-55535.
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I am using CD83 (F-5): sc-55535 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Worked well with CD83 non Worked well with CD83 non-transfected and transfected K-562 whole cell lysates. -SCBT QC
Date published: 2015-07-01
Rated 5 out of 5 by from Produced excellent immunoperoxidase membrane Produced excellent immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2015-01-28
Rated 5 out of 5 by from Exceptional Indirect FCM data of K Exceptional Indirect FCM data of K-562-human CD83 stable transfectant cell line stained with CD83 . -SCBT QC
Date published: 2014-11-27
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