CD74 Antibody (OX6)recommended for detection of CD74 of mouse and rat origin by WB, IF, IHC(P) and FCM

CD74 Antibody (OX6): sc-53062

CD74 Antibody (OX6) is rated 4.8 out of 5 by 5.
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Datasheets

    • CD74 Antibody (OX6) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against RT1b monomorphic/mouse -I-Ak,s of rat origin
    • recommended for detection of CD74 of mouse and rat origin by WB, IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See CD74 (LN-2): sc-6262 for CD74 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can CD74 (OX6): sc-53062 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD74 (OX6): sc-53062 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great for Flow Cytometry Excellent choice for CD74, gives nice Flow cytometry data!
Date published: 2017-02-03
Rated 5 out of 5 by from Publishable IHC data in rat spinal cord tissue Publishable IHC data in rat spinal cord tissue. -SCBT Publication Review
Date published: 2015-06-01
Rated 5 out of 5 by from Publishable data obtained by WB using mouse Publishable data obtained by WB using mouse IKDC and NK from spleen. -SCBT Publication Review
Date published: 2015-05-19
Rated 5 out of 5 by from It has been reported that product sc It has been reported that product sc-53062 is suitable for use in IF using rat brain and dentate gyrus tissue. -SCBT Publication Review
Date published: 2015-01-27
Rated 4 out of 5 by from Produced robust indirect FCM data for rat Produced robust indirect FCM data for rat peripheral blood leukocytes stained CD74 . -SCBT QC
Date published: 2014-11-15
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