CD74 Antibody (By2)recommended for detection of CD74 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

CD74 Antibody (By2): sc-20062

CD74 Antibody (By2) is rated 4.2 out of 5 by 5.
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Datasheets

    • CD74 Antibody (By2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against B-cell lymphoma cells
    • recommended for detection of CD74 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin, FITC, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 405 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See CD74 (LN-2): sc-6262 for CD74 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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How can CD74 (By2): sc-20062 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Professor Griffin
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-20062 PE) or fluoroscein (sc-20062 FITC).
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from It Works It works good and specific in IHC with human tissues.
Date published: 2017-01-10
Rated 2 out of 5 by from Robust band seen in WB using NAMALWA whole Robust band seen in WB using NAMALWA whole cell lysate. -SCBT QC
Date published: 2015-06-02
Rated 5 out of 5 by from Superb immunoperoxidase membrane and cytoplasmic Superb immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human spleen tissue. -SCBT QC
Date published: 2015-05-10
Rated 4 out of 5 by from Nice bands seen in WB using 293T and HeLa Nice bands seen in WB using 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2015-03-18
Rated 5 out of 5 by from Exceptional intracellular FCM data of fixed Exceptional intracellular FCM data of fixed and permeabilized BJAB cells. -SCBT QC
Date published: 2014-03-13
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