CD74 Antibody (A-6)recommended for detection of CD74 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

CD74 Antibody (A-6): sc-166047

CD74 Antibody (A-6) is rated 4.3 out of 5 by 4.
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Datasheets

    • CD74 Antibody (A-6) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 17-296 mapping at the C-terminus of CD74 of human origin
    • recommended for detection of CD74 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See CD74 (LN-2): sc-6262 for CD74 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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I am using CD74 (A-6): sc-166047 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Date published: 2015-06-26
Rated 3 out of 5 by from Robust band seen in WB using BJAB whole Robust band seen in WB using BJAB whole cell lysate. -SCBT QC
Date published: 2015-06-20
Rated 5 out of 5 by from Published IHC data with human Brain Published IHC data with human Brain - SCBT Publication Review
Date published: 2015-05-18
Rated 5 out of 5 by from Exceptional immunofluorescence cytoplasmic Exceptional immunofluorescence cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2014-05-02
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