Anti-CD45RO Antibody (UCH-L1) recommended for detection of CD45RO (180-185 kDa) of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

Anti-CD45RO Antibody (UCH-L1): sc-1183

Anti-CD45RO Antibody (UCH-L1) is rated 4.9 out of 5 by 8.
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Datasheets

    • Anti-CD45RO Antibody (UCH-L1) is a mouse monoclonal IgG2a κ CD45RO antibody, cited in 12 publications, provided at 200 µg/ml
    • raised against cultured human T cell line-CA-1
    • Anti-CD45RO Antibody (UCH-L1) is recommended for detection of CD45RO (180-185 kDa) of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • Anti-CD45RO Antibody (UCH-L1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to APC for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD45RO (UCH-L1): sc-1183.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for CD45RO Antibody (UCH-L1) for WB and IHC(P) applications. These reagents are now offered in bundles with CD45RO Antibody (UCH-L1) (see ordering information below).
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Which negative control should be used when using the PE conjugated CD45RO (UCH-L1): sc-1183 monoclonal antibody (sc-1183 PE) in flow cytometry?

Asked by: TinTin
Thank you for your inquiry. We recommend using isotype specific normal mouse IgG2a conjugated to PE, sc-2867.
Answered by: Tech Service
Date published: 2017-02-02
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Rated 5 out of 5 by from Versitle Antibody Very nice IHC (P), IF, WB, and FCM data obtained with CD45RO (UCH-L1): sc-1183 monoclonal antibody
Date published: 2017-01-05
Rated 5 out of 5 by from Function of cd45ro is very good in fcm IN FCM, CD45RO IS USED TO STAIN WB TO FIND HELPER T CELLS. Function is very good.
Date published: 2016-11-20
Rated 5 out of 5 by from IF is good IF is good
Date published: 2015-10-16
Rated 5 out of 5 by from Produced great FCM data for CCRF Produced great FCM data for CCRF-CEM cells. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from Good data with paraffin Good data with paraffin-embedded human Lymph Node. -SCBT Publication Review
Date published: 2015-05-02
Rated 4 out of 5 by from Robust WB band obtained using against Jurkat Robust WB band obtained using against Jurkat whole cell lysate. -SCBT QC
Date published: 2015-05-02
Rated 5 out of 5 by from Beautiful immunoperoxidase cytoplasmic and Beautiful immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human spleen tissue. -SCBT QC
Date published: 2015-03-08
Rated 5 out of 5 by from Produced superb Indirect FCM data for CCRF Produced superb Indirect FCM data for CCRF-CEM stained with CD45RO . -SCBT QC
Date published: 2013-03-08
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