Anti-CD45RC Antibody (6A295) recommended for detection of CD45RC of mouse and rat origin by WB, IP, IF, IHC(P) and FCM

Anti-CD45RC Antibody (6A295): sc-70711

Anti-CD45RC Antibody (6A295) is rated 4.0 out of 5 by 1.
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Datasheets

    • CD45RC Antibody (6A295) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against stimulated lymphocytes of rat origin
    • recommended for detection of CD45RC of mouse and rat origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD45RC Antibody (6A295). This work is in progress.
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How much sample do you recommend loading on the gel for Western blot detection using CD45RC (6A295): sc-70711?

Asked by: Cweed
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Strong signal in WB in rat PBL whole cell lysate Strong signal in WB in rat PBL whole cell lysate. -SCBT QC
Date published: 2014-05-23
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