Anti-CD45RB Antibody (BRA11) recommended for detection of CD45RB of human origin by WB, IP, IF and IHC(P)

Anti-CD45RB Antibody (BRA11): sc-66202

Anti-CD45RB Antibody (BRA11) is rated 5.0 out of 5 by 2.
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Datasheets

    • CD45RB Antibody (BRA11) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against CALLA positive acute lymphoid lukemia cell line REH of human origin
    • CD45RB Antibody (BRA11) is recommended for detection of CD45RB of human origin by WB, IP, IF and IHC(P)
    • Anti-CD45RB Antibody (BRA11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD45RB (BRA11): sc-66202.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD45RB Antibody (BRA11) for WB and IHC(P) applications. These reagents are now offered in bundles with CD45RB Antibody (BRA11) (see ordering information below).
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How can CD45RB (BRA11): sc-66202 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: TinTin
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-66202 PE) or fluoroscein (sc-66202 FITC).
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Very robust signal Very robust signal, some background observed, in WB in GA-10, CCRF-CEM, U-698-M and MOLT-4 whole cell lysates. -SCBT QC
Date published: 2015-04-26
Rated 5 out of 5 by from Produced great Indirect FCM data for human Produced great Indirect FCM data for human peripheral blood leukocytes stained with CD45RB . -SCBT QC
Date published: 2014-11-11
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