Anti-CD45R Antibody (RA3-6B2) recommended for detection of CD45R of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

Anti-CD45R Antibody (RA3-6B2): sc-19597

Anti-CD45R Antibody (RA3-6B2) is rated 4.4 out of 5 by 11.
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Datasheets

    • Anti-CD45R Antibody (RA3-6B2) is a rat monoclonal IgG2a CD45R antibody, cited in 22 publications, provided at 200 µg/ml
    • Anti-CD45R Antibody (RA3-6B2) is recommended for detection of CD45R of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • Anti-CD45R Antibody (RA3-6B2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either APC-Cy7 or PerCP for IF, IHC(P) and FCM
    • also provided azide-free for modulation of B-cell responses in vitro and in vivo, sc-19597 L, 200 µg/0.1 ml
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD45R (RA3-6B2): sc-19597.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD45R Antibody (RA3-6B2). This work is in progress.
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What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-02-09
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Rated 3 out of 5 by from Positive The immunohistochemistry was conducted on human autoptic lung tissue and we obtained a slight cytoplasmic staining.
Date published: 2021-02-22
Rated 1 out of 5 by from Did not work on canine samples (IHC-P) Tried on different canine tissues with different protocols, but I did not observe reactivity on FFPE samples. (IHC-P)
Date published: 2020-07-16
Rated 4 out of 5 by from Gute Ergebnisse in der IF Wir haben eine Probepackung erhalten. Der Antikörper hat ein gutes Signal in der IF von gefrorenen Gewebeschnitten gezeigt.
Date published: 2018-03-01
Rated 5 out of 5 by from publish using It works of mouse spleen tissue, the band is specific and clear.
Date published: 2017-05-19
Rated 5 out of 5 by from Provides good reactivity. This CD45R antibody reacts well with mouse, rat, and human samples. It will provide a strong signal in WB and clear staining in IHC and IF. It has been successfully published with.
Date published: 2017-01-19
Rated 5 out of 5 by from Worked very well in IHC(P) in mouse Worked very well in IHC(P) in mouse
Date published: 2015-09-04
Rated 5 out of 5 by from This antibody worked very well on IHC(P) This antibody worked very well on IHC(P)
Date published: 2015-08-04
Rated 5 out of 5 by from Produced excellent immunoperoxidase membrane Produced excellent immunoperoxidase membrane staining in formalin-fixed mouse peripheral blood lymphocytes. -SCBT QC
Date published: 2015-05-17
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