Anti-CD45 Antibody (YTH24.5) recommended for detection of CD45 of human origin by WB, IP and FCM

Anti-CD45 Antibody (YTH24.5): sc-59070

Anti-CD45 Antibody (YTH24.5) is rated 4.0 out of 5 by 3.
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Datasheets

    • CD45 Antibody (YTH24.5) is a rat monoclonal IgG2b, cited in 1 publications, provided at 200 µg/ml
    • raised against T lymphocytes of human origin
    • CD45 Antibody (YTH24.5) is recommended for detection of CD45 of human origin by WB, IP and FCM
    • Anti-CD45 Antibody (YTH24.5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD45 (YTH24.5): sc-59070.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD45 Antibody (YTH24.5). This work is in progress.
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Can CD45 (YTH24.5): sc-59070 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD45 (YTH24.5): sc-59070 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Strong band in WB in CCRF Strong band in WB in CCRF-CEM whole cell lysate. -SCBT QC
Date published: 2015-04-19
Rated 4 out of 5 by from Positive band visualized in WB in NAMALWA Positive band visualized in WB in NAMALWA, Ramos, AML-193 and HL-60 whole cell lysates. -SCBT QC
Date published: 2015-02-05
Rated 4 out of 5 by from Robust Indirect FCM data of human peripheral Robust Indirect FCM data of human peripheral blood leukocytes stained with CD45 . -SCBT QC
Date published: 2014-08-02
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