Anti-CD45 Antibody (OX30) recommended for detection of a monomorphic determinant of CD45 of mouse and rat origin by WB, IP, IF, IHC(P) and FCM

Anti-CD45 Antibody (OX30): sc-53047

Anti-CD45 Antibody (OX30) is rated 4.3 out of 5 by 4.
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Datasheets

    • CD45 Antibody (OX30) is a mouse monoclonal IgG2a κ, cited in 13 publications, provided at 200 µg/ml
    • raised against lymph node glycoproteins and cells of rat origin
    • Anti-CD45 Antibody (OX30) is recommended for detection of a monomorphic determinant of CD45 of mouse and rat origin by WB, IP, IF, IHC(P) and FCM
    • Anti-CD45 Antibody (OX30) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD45 (OX30): sc-53047.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for CD45 Antibody (OX30) for WB and IHC(P) applications. These reagents are now offered in bundles with CD45 Antibody (OX30) (see ordering information below).
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If I want to use this antibody for Flow Cytometry, what starting dilution do you recommend I use?

Asked by: cjMara
We recommend a starting dilution of 1µg of antibody per million cells. Here is a link to our recommended protocol. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support 7
Date published: 2017-01-25
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Rated 4 out of 5 by from 很好,一次成功 几个月前从朋友那借了点抗体,效果很好,没想到第一次做,就出阳性结果了!真的很开心,也非常感谢您们公司!
Date published: 2019-05-27
Rated 5 out of 5 by from Great for Flow Cytometry Positive results obtain with CD45 Antibody (OX30) by Flow cytometry as well as WB
Date published: 2017-01-17
Rated 4 out of 5 by from Single band with some background for WB in Single band with some background for WB in rat PBL whole cell lysate. -SCBT QC
Date published: 2015-04-03
Rated 4 out of 5 by from Produced good Indirect FCM data for rat peripheral Produced good Indirect FCM data for rat peripheral blood leukocytes stained with CD45 . -SCBT QC
Date published: 2014-12-15
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