Anti-CD45 Antibody (OX1) recommended for detection of monomorphic determinant of CD45 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA

Anti-CD45 Antibody (OX1): sc-53045

Anti-CD45 Antibody (OX1) is rated 4.0 out of 5 by 2.
  • y_2022, m_5, d_21, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.27
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_53045, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 118ms
  • REVIEWS, PRODUCT
loading
5
0
4
2
3
0
2
0
1
0

Datasheets

    • CD45 Antibody (OX1) is a mouse monoclonal IgG1 κ, cited in 10 publications, provided at 200 µg/ml
    • raised against full length thymocyte membrane glycoproteins of rat origin
    • recommended for detection of monomorphic determinant of CD45 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See CD45 (35-Z6): sc-1178 for CD45 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD45 Antibody (OX1) for WB applications. These reagents are now offered in bundles with CD45 Antibody (OX1) (see ordering information below).
loading

Can CD45 (OX1): sc-53045 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD45 (OX1): sc-53045 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
  • y_2022, m_5, d_21, h_21CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.27
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_53045, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 101ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from FACS publication in rat heart samples FACS publication in rat heart samples. -SCBT Publication Review
Date published: 2015-04-24
Rated 4 out of 5 by from Produced robust Indirect FCM data for rat Produced robust Indirect FCM data for rat peripheral blood leukocytes stained with CD45 . -SCBT QC
Date published: 2014-07-26
  • y_2022, m_5, d_21, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.27
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_53045, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 16ms
  • REVIEWS, PRODUCT