Anti-CD45 Antibody (3H1363) recommended for detection of CD45 of human origin by WB, IP and FCM

Anti-CD45 Antibody (3H1363): sc-70699

Anti-CD45 Antibody (3H1363) is rated 5.0 out of 5 by 3.
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Datasheets

    • CD45 Antibody (3H1363) is a rat monoclonal IgG2b, cited in 6 publications, provided at 200 µg/ml
    • raised against T lymphocytes of human origin
    • recommended for detection of CD45 of human origin by WB, IP and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See CD45 (35-Z6): sc-1178 for CD45 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD45 Antibody (3H1363). This work is in progress.
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I would like to use CD45 Antibody (3H1363): sc-70699 for IP applications. Does this antibody come pre-conjugated to agarose?

Asked by: Trav11
Thank you for your question. Please send an email request to [email protected] for primary antibody conjugation not listed on our website. Also, you may want to consider using CD45 (35-Z6): sc-1178 as it is currently available conjugated to agarose, HRP, FITC, PE, Alexa Fluor 488, and Alexa Fluor 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 3
Date published: 2017-01-11

What is the recommended positive control for CD45 Antibody (3H1363): sc-70699?

Asked by: Trav11
Thank you for your question. For CD45 Antibody (3H1363): sc-70699, there are several options for positive controls, such as Jurkat Whole Cell Lysate (sc-2204) or HL-60 Whole Cell Lysate (sc-2209). If you have any further questions or concerns, or would like to know additional positive controls for sc-70699, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 3
Date published: 2017-01-11
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Rated 5 out of 5 by from Great WB Very nice western blot data; strong signal with low background
Date published: 2017-01-15
Rated 5 out of 5 by from Great for WB Excellent band strength and clean background in Western Blot with human whole cell lysates.
Date published: 2016-12-23
Rated 5 out of 5 by from Works well for western blot; tested with GA Works well for western blot; tested with GA-10, human PBL and Ramos whole cell lysates. -SCBT QC
Date published: 2014-09-26
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