Anti-CD45 Antibody (2B11) recommended for detection of CD45 of human origin by WB, IP, IF, IHC(P) and FCM

Anti-CD45 Antibody (2B11): sc-20056

Anti-CD45 Antibody (2B11) is rated 4.0 out of 5 by 3.
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Datasheets

    • CD45 Antibody (2B11) is a mouse monoclonal IgG1 κ, cited in 5 publications, provided at 200 µg/ml
    • raised against isolated neoplastic cells from a T cell lymphoma
    • recommended for detection of CD45 of human origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See CD45 (35-Z6): sc-1178 for CD45 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD45 Antibody (2B11) for WB and IHC(P) applications. These reagents are now offered in bundles with CD45 Antibody (2B11) (see ordering information below).
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Can CD45 (2B11): sc-20056 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD45 (2B11): sc-20056 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Clear immunofluorescence membrane staining Clear immunofluorescence membrane staining in methanol-fixed GA-10 cells. -SCBT QC
Date published: 2015-05-01
Rated 4 out of 5 by from WB: robust bands present in CCRF WB: robust bands present in CCRF-CEM, GA-10 and Ramos whole cell lysates. -SCBT QC
Date published: 2015-03-18
Rated 4 out of 5 by from Produced great FCM data for human peripheral Produced great FCM data for human peripheral blood leukocytes. -SCBT QC
Date published: 2014-06-10
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