Anti-CD4 Antibody (RM4-5) recommended for detection of CD4 of mouse origin by WB, IP, IF and FCM

Anti-CD4 Antibody (RM4-5): sc-19643

Anti-CD4 Antibody (RM4-5) is rated 3.8 out of 5 by 4.
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Datasheets

    • CD4 Antibody (RM4-5) is a rat monoclonal IgG2a, cited in 7 publications, provided at 200 µg/ml
    • raised against BALB/c mouse thymocytes
    • recommended for detection of CD4 of mouse origin by WB, IP, IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to PerCP for IF, IHC(P) and FCM
    • See CD4 (MT310): sc-19641 for CD4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD4 Antibody (RM4-5). This work is in progress.
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Can CD4 (RM4-5): sc-19643 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD4 (RM4-5): sc-19643 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good publishable data in IHC in mouse spleen Good publishable data in IHC in mouse spleen and lymph node. -SCBT Publication Review
Date published: 2015-05-09
Rated 4 out of 5 by from Robust signal in WB in mouse transfected Robust signal in WB in mouse transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-02-14
Rated 4 out of 5 by from Published from flow in mouse cryostat KC cells Published from flow in mouse cryostat KC cells. -SCBT Publication Review
Date published: 2015-01-09
Rated 3 out of 5 by from Decent FCM data of mouse peripheral blood Decent FCM data of mouse peripheral blood leukocytes. -SCBT QC
Date published: 2013-09-03
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