Anti-CD4 Antibody (OX68) recommended for detection of CD4 domains 3 and 4 of mouse, rat and human origin by IF, IHC(P) and FCM

Anti-CD4 Antibody (OX68): sc-53042

Anti-CD4 Antibody (OX68) is rated 3.0 out of 5 by 1.
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Datasheets

    • CD4 Antibody (OX68) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
    • raised against purified CD4 of rat origin
    • recommended for detection of CD4 domains 3 and 4 of mouse, rat and human origin by IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to PerCP for IF, IHC(P) and FCM
    • See CD4 (MT310): sc-19641 for CD4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for CD4 Antibody (OX68) for IHC(P) applications. These reagents are now offered in bundles with CD4 Antibody (OX68) (see ordering information below).
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I am using CD4 (OX68): sc-53042 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-04-05
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Rated 3 out of 5 by from Produced decent Indirect FCM data for rat Produced decent Indirect FCM data for rat peripheral blood leukocytes stained with CD4 . -SCBT QC
Date published: 2013-02-26
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