CD4 Antibody (MT310) recommended for detection of CD4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

CD4 Antibody (MT310): sc-19641

CD4 Antibody (MT310) is rated 4.0 out of 5 by 17.
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Datasheets

    • Anti-CD4 Antibody (MT310) is a mouse monoclonal IgG1 κ CD4 antibody, cited in 41 publications, provided at 200 µg/ml
    • Anti-CD4 Antibody (MT310) is recommended for detection of CD4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • Anti-CD4 Antibody (MT310) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either APC-Cy7, PE-Cy7, PerCP, PerCP-Cy5.5 or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD4 (MT310): sc-19641.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for CD4 Antibody (MT310) for WB and IHC(P) applications. These reagents are now offered in bundles with CD4 Antibody (MT310) (see ordering information below).

Our laboratory has been performing western blots using human CD4 and we obtained this antibody but have seen cross reactivity with our other antibody that binds human CD4. Our other antibody is 6-HIS tagged and contains mouse heavy and light chains.

Asked by: CitlaV
Thank you for your question. This CD4 Antibody (MT310) is a mouse monoclonal IgG1 (kappa light chain). It is recommended for detection of CD4 of mouse, rat and human origin. It should not cross-react with other mouse monoclonal primary antibodies, or mouse IgG. If your other antibody is also a mouse monoclonal IgG, then we recommend including a secondary antibody control on your Western blot (omit primary antibody). This will help evaluate potential cross-reactivity of your secondary antibody (anti-mouse IgG) with your samples. Please contact us for further Technical Support.
Answered by: Technical Support
Date published: 2020-01-15

I will use the anti-mouse Ab CD4 (MT 310) -HRP sc-19641. At what concentration can I use it for a Western blot and in which buffer for blocking and incubation. Thank you

Asked by: ABIOUMI
We recommend to use the primary antibody at a dilution of 1:200 for Western blotting. We recommend using 5% nonfat milk + TBST for blocking and antibody incubation. Below is a link to our recommended protocol for more detailed information. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2018-11-20

What is the concentration provided for CD4 (MT310): sc-19641 PerCP and CD4 (MT310): sc-19641 Alexa Fluor® 405?

Asked by: jenni
The concentration provided for CD4 (MT310): sc-19641 PerCP and CD4 (MT310): sc-19641 Alexa Fluor® 405 is 50 ug/mL.
Answered by: Technical Support 12
Date published: 2017-01-09
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Rated 4 out of 5 by from Clear and clean bands I bought this two years ago. I tested it on samples of rat lymphocytes and got a clear band at 55 KDa
Date published: 2019-02-25
Rated 3 out of 5 by from CD4用于组化检测 很高兴能给试用,但是组化效果并不是很理想,最后全把试用的量加进去了,勉强有点信号,不知是试用装的原因还是其他原因
Date published: 2017-12-08
Rated 3 out of 5 by from No satisfactory results I applied for 5 trial antibodies a month and a half ago, and three of them achieved satisfactory results, but unfortunately this antibody may not be applicable to our samples,rat spleen,Immunohistochemistry was performed at 1:50, 1:100 paraffin sections.
Date published: 2017-09-05
Rated 5 out of 5 by from Excellent resuts Last month ,I signed up the sample of CD4 antibody on SANTA CRUZ as for my preliminary experiment ,the result was excellent .
Date published: 2017-07-04
Rated 4 out of 5 by from work ok in immunofluorescence This antibody works in immunofluorescence of tissue frozen sections, but is weak.
Date published: 2017-06-08
Rated 5 out of 5 by from Blot clean Clear band observed by WB using CCRF-CEM whole cell lysate, no background
Date published: 2017-06-02
Rated 5 out of 5 by from 效果很好 前不久,我用了这个抗体,图片结果很好,背景还是比较干净,非特异性染色较少,以后会继续光顾的。希望santa越做越好。
Date published: 2017-02-27
Rated 4 out of 5 by from Nice product Pleased with both western blot and IHC results; also fmc
Date published: 2017-01-07
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