Anti-CD4 Antibody (MT310) recommended for detection of CD4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

Anti-CD4 Antibody (MT310): sc-19641

Anti-CD4 Antibody (MT310) is rated 4.1 out of 5 by 19.
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Datasheets

    • Anti-CD4 Antibody (MT310) is a mouse monoclonal IgG1 κ CD4 antibody, cited in 48 publications, provided at 200 µg/ml
    • Anti-CD4 Antibody (MT310) is recommended for detection of CD4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • Anti-CD4 Antibody (MT310) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either APC-Cy7, PerCP or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD4 (MT310): sc-19641.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD4 Antibody (MT310) for WB and IHC(P) applications. These reagents are now offered in bundles with CD4 Antibody (MT310) (see ordering information below).
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I need an anti human CD4 antibody specific for D1 or D2 for Western blot. Will the MT310 work for my purpose? Also, does it cross reacts with monkey CD4? Thanks!

Asked by: LilyA
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions
Answered by: Blake J
Date published: 2021-10-11

能否应用于大鼠睾丸石蜡切片的免疫荧光

Asked by: 学渣zz
感谢您的提问。CD4 抗体 (MT310): sc-19641 推荐用于大鼠石蜡切片的免疫荧光,但是我们没有在大鼠睾丸组织中验证过。 Thank you for your question. Anti-CD4 Antibody (MT310): sc-19641 is recommended for paraffin embedded rat species on IF, but we didn't validate it on paraffin embedded rat testis tissue.
Answered by: Technical Support 12
Date published: 2021-07-06

Our laboratory has been performing western blots using human CD4 and we obtained this antibody but have seen cross reactivity with our other antibody that binds human CD4. Our other antibody is 6-HIS tagged and contains mouse heavy and light chains.

Asked by: CitlaV
Thank you for your question. This CD4 Antibody (MT310) is a mouse monoclonal IgG1 (kappa light chain). It is recommended for detection of CD4 of mouse, rat and human origin. It should not cross-react with other mouse monoclonal primary antibodies, or mouse IgG. If your other antibody is also a mouse monoclonal IgG, then we recommend including a secondary antibody control on your Western blot (omit primary antibody). This will help evaluate potential cross-reactivity of your secondary antibody (anti-mouse IgG) with your samples. Please contact us for further Technical Support.
Answered by: Technical Support
Date published: 2020-01-15

I will use the anti-mouse Ab CD4 (MT 310) -HRP sc-19641. At what concentration can I use it for a Western blot and in which buffer for blocking and incubation. Thank you

Asked by: ABIOUMI
We recommend to use the primary antibody at a dilution of 1:200 for Western blotting. We recommend using 5% nonfat milk + TBST for blocking and antibody incubation. Below is a link to our recommended protocol for more detailed information. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2018-11-20

What is the concentration provided for CD4 (MT310): sc-19641 PerCP and CD4 (MT310): sc-19641 Alexa Fluor® 405?

Asked by: jenni
The concentration provided for CD4 (MT310): sc-19641 PerCP and CD4 (MT310): sc-19641 Alexa Fluor® 405 is 50 ug/mL.
Answered by: Technical Support 12
Date published: 2017-01-09
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Rated 5 out of 5 by from 抗体不错。 这是SD大鼠睾丸的石蜡切片免疫荧光,非常不错的抗体,一次就做成功了。此外,我们通过细胞测序发现多种可能染色的marker,如果有可能,希望贵公司可以多提供几种小样供测试,如果有好的结果也会反馈给贵公司。
Date published: 2021-07-15
Rated 5 out of 5 by from 功能很出色! 我一个月前购买了此产品,质量相当不错,很多其他抗体公司的抗体都染不出来,这个可以,本人极力推荐,赞赞赞
Date published: 2021-04-14
Rated 4 out of 5 by from Clear and clean bands I bought this two years ago. I tested it on samples of rat lymphocytes and got a clear band at 55 KDa
Date published: 2019-02-25
Rated 3 out of 5 by from CD4用于组化检测 很高兴能给试用,但是组化效果并不是很理想,最后全把试用的量加进去了,勉强有点信号,不知是试用装的原因还是其他原因
Date published: 2017-12-08
Rated 3 out of 5 by from No satisfactory results I applied for 5 trial antibodies a month and a half ago, and three of them achieved satisfactory results, but unfortunately this antibody may not be applicable to our samples,rat spleen,Immunohistochemistry was performed at 1:50, 1:100 paraffin sections.
Date published: 2017-09-05
Rated 5 out of 5 by from Excellent resuts Last month ,I signed up the sample of CD4 antibody on SANTA CRUZ as for my preliminary experiment ,the result was excellent .
Date published: 2017-07-04
Rated 4 out of 5 by from work ok in immunofluorescence This antibody works in immunofluorescence of tissue frozen sections, but is weak.
Date published: 2017-06-08
Rated 5 out of 5 by from Blot clean Clear band observed by WB using CCRF-CEM whole cell lysate, no background
Date published: 2017-06-02
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