Anti-CD4 Antibody (H129.19) recommended for detection of CD4 of mouse origin by IP, IF and FCM

Anti-CD4 Antibody (H129.19): sc-19642

Anti-CD4 Antibody (H129.19) is rated 4.0 out of 5 by 1.
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Datasheets

    • CD4 Antibody (H129.19) is a rat monoclonal IgG2a, cited in 4 publications, provided at 200 µg/ml
    • raised against A.TH. mouse CTL clone A15.1.17
    • recommended for detection of CD4 of mouse origin by IP, IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to APC for IF, IHC(P) and FCM
    • also provided azide-free for blocking T helper cell responses to antigen or mitogen stimulation, sc-19642 L, 200 µg/0.1 ml
    • See CD4 (MT310): sc-19641 for CD4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD4 Antibody (H129.19). This work is in progress.
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Does this antibody work in IHC of sections from paraffin-embedded samples? Thanks for your help, Staffan

Asked by: Staffan
Thank you for your question. For staining paraffin-embedded, formalin-fixed tissue sample sections we would rather recommend using one of the two following alternate CD4 antibodies. We have several pieces of data and product citations for these products in the entiond application IHC(P). CD4 (MT310): sc-19641 https://www.scbt.com/scbt/product/cd4-antibody-mt310 CD4 (GK1.5): sc-13573 https://www.scbt.com/scbt/product/cd4-antibody-gk1-5 We suggest using the directly -HRP conjugated primary antibody according to our protocols: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining if you have any futher questions, please contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

Does H129-19 work in IHC for sections from paraffin-imbedded samples? Staffan

Asked by: Staffan
Thank you for your question. For staining paraffin-embedded, formalin-fixed tissue sample sections we would rather recommend using one of the two following alternate CD4 antibodies. We have several pieces of data and product citations for these products in the entiond application IHC(P). CD4 (MT310): sc-19641 https://www.scbt.com/scbt/product/cd4-antibody-mt310 CD4 (GK1.5): sc-13573 https://www.scbt.com/scbt/product/cd4-antibody-gk1-5 We suggest using the directly -HRP conjugated primary antibody according to our protocols: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining if you have any futher questions, please contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

How can CD4 (H129.19): sc-19642 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: TinTin
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-19642 PE) or fluoroscein (sc-19642 FITC).
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Wonderful FCM analysis of mouse peripheral Wonderful FCM analysis of mouse peripheral blood leukocytes. -SCBT QC
Date published: 2013-09-11
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