Anti-CD4 Antibody (GK1.5) recommended for detection of an extracellular region of CD4 of mouse, rat and human origin by IP, IF, IHC(P) and FCM

Anti-CD4 Antibody (GK1.5): sc-13573

Anti-CD4 Antibody (GK1.5) is rated 4.1 out of 5 by 15.
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Datasheets

    • CD4 Antibody (GK1.5) is a rat monoclonal IgG2b, cited in 50 publications, provided at 200 µg/ml
    • raised against CTL clone V4 cells of mouse origin
    • Anti-CD4 Antibody (GK1.5) is recommended for detection of an extracellular region of CD4 of mouse, rat and human origin by IP, IF, IHC(P) and FCM
    • CD4 Antibody (GK1.5) is available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 405 for IF, IHC(P) and FCM
    • available azide-free for blocking of T cell activation and ligand binding (sc-13573 L, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD4 (GK1.5): sc-13573.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD4 Antibody (GK1.5). This work is in progress.
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Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-13573 PE), fluoroscein (sc-13573 FITC), Alexa Fluor® 488 (sc-13573 AF488) or Alexa Fluor® 647 (sc-13573 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-30
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Rated 1 out of 5 by from Did not work Tried on different tissues (from canine and equine species) with different protocols, but I did not observe reactivity on FFPE samples. (IHC-P)
Date published: 2020-07-16
Rated 5 out of 5 by from Highly specific antibodies Mouse spleen Blue-DAPI Green-IL-1b: sc-520 (1:50) Magenta-CD4: sc13573 (1:50) 4 hrs incubation RT
Date published: 2018-03-02
Rated 1 out of 5 by from did not work I had it as a free sample and tried right away. but it did not work. It did not stain Cd4+ cells in a FFPE lung sections.
Date published: 2017-12-21
Rated 5 out of 5 by from Great I used it for Western Blot and Flow Cytometry and got good results.
Date published: 2017-10-30
Rated 5 out of 5 by from 抗体不错 几个月前买的抗体,老师很开心,结果很好,非常感谢scbt的好抗体。希望公司能一如既往的保持好品质。谢谢
Date published: 2017-10-25
Rated 5 out of 5 by from 抗体不错,老板满意 开心,实验出了结果。This excellent antibody has great reactivity in a wide range of applications. It demonstrates strong bands in a Western blot and has provide great flow cytometry data. This product has been published over 70 times!
Date published: 2017-10-09
Rated 4 out of 5 by from Great features! The antibody is work well in WB at 1:100,skeletal muscle cells.
Date published: 2017-09-01
Rated 5 out of 5 by from Great for all applications! Excellent performance in WB, IHC and Flow, as well as being validated for T cell activation and ligand binding.
Date published: 2017-01-16
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