Anti-CD4 Antibody (EDU-2) recommended for detection of CD4 of human origin by FCM

Anti-CD4 Antibody (EDU-2): sc-51544

Anti-CD4 Antibody (EDU-2) is rated 3.0 out of 5 by 2.
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Datasheets

    • CD4 Antibody (EDU-2) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 200 µg/ml
    • raised against human T-lymphocytes
    • recommended for detection of CD4 of human origin by FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See CD4 (MT310): sc-19641 for CD4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for CD4 Antibody (EDU-2) for WB and IHC(P) applications. These reagents are now offered in bundles with CD4 Antibody (EDU-2) (see ordering information below).
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How can CD4 (EDU-2): sc-51544 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Randy McDonald
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-51544 PE) or fluoroscein (sc-51544 FITC).
Answered by: Technical Support
Date published: 2017-03-01
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Rated 3 out of 5 by from Produced good Indirect FCM data for human Produced good Indirect FCM data for human peripheral blood leukocytes stained with CD4 . -SCBT QC
Date published: 2015-06-01
Rated 3 out of 5 by from Produced decent FCM data for HuT 78 cells Produced decent FCM data for HuT 78 cells. -SCBT QC
Date published: 2014-04-05
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