Anti-CD4 Antibody (A-1) recommended for detection of CD4 of human origin by WB, IP, IF and ELISA

Anti-CD4 Antibody (A-1): sc-514571

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Datasheets

    • CD4 Antibody (A-1) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 26-396 of CD4 of human origin
    • recommended for detection of CD4 of human origin by WB, IP, IF and ELISA
    • See CD4 (MT310): sc-19641 for CD4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD4 Antibody (A-1) for WB applications. These reagents are now offered in bundles with CD4 Antibody (A-1) (see ordering information below).
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Does your CD4 Antibody (A-1): sc-514571 also react with human TH1 ?

Asked by: Prof Charles
Thank you for your question. CD4 Antibody (A-1): sc-514571 was raised against amino acids 26-396 of CD4 of human origin and is recommended for detection of CD4 of human origin by WB, IP, IF and ELISA. It is not known to detect TH1. Please refer to our antibodies specific against NELF (TH1 synonym) in order to detect human TH1. You can find an overview of these antibodied by following this link: https://www.scbt.com/scbt/browse/nelf-Antibodies/_/N-1lrpqub Please contact technical service directly if you have further questions.
Answered by: Technical Support Europe
Date published: 2019-04-26

What is the recommended starting dilution for Western blot for sc-514571: CD4 (A-1) monoclonal antibody?

Asked by: Germaine
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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