Anti-CD4 Antibody (6A17) recommended for detection of CD4 of human origin by WB, IP, IF and IHC(P)

Anti-CD4 Antibody (6A17): sc-70659

Anti-CD4 Antibody (6A17) is rated 4.5 out of 5 by 2.
  • y_2021, m_12, d_6, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_70659, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 103ms
  • REVIEWS, PRODUCT
loading
5
1
4
1
3
0
2
0
1
0

Datasheets

    • CD4 Antibody (6A17) is a mouse monoclonal IgG1, cited in 1 publications, provided at 250 µl supernatant
    • raised against the extracellular domain of CD4 of human origin
    • recommended for detection of CD4 of human origin by WB, IP, IF and IHC(P)
    • See CD4 (MT310): sc-19641 for CD4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD4 Antibody (6A17). This work is in progress.
loading

I would like to use CD4 (6A17): sc-70659 for immunoprecipitation. Which protein agarose do you recommend using with this antibody?

Asked by: cjMara
Thank you for your question. We recommend using Protein A/G PLUS-Agarose: sc-2003 https://www.scbt.com/scbt/product/protein-a-g-plus-agarose
Answered by: Technical Support
Date published: 2017-04-12

Hello, I would like to know whether the anti-CD4 antibody (6A17) cat no sc-70659 needs a heat-mediated antigen retrieval for IHC-paraffin and if so what pH is recommended. Thank you

Asked by: Leonarda
Thank you for your inquiry. Yes, we do recommend antigen retrieval for IHC with paraffin-embedded sections. Heat treatment (recommended method): Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0; or with 50 mM glycine-HCl buffer (glycine: sc-29096), pH 3.5, with 0.01% (w/v) EDTA (EDTA: sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides. You can view our full protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Service
Date published: 2016-11-02
  • y_2021, m_12, d_6, h_19CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_70659, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 111ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Date published: 2015-03-31
Rated 5 out of 5 by from Works well for WB with SUP Works well for WB with SUP-T1 and CCRF-CEM whole cell lysates. -SCBT QC
Date published: 2013-10-13
  • y_2021, m_12, d_6, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_70659, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT