CD4 Antibody (6A17) recommended for detection of CD4 of human origin by WB, IP, IF and IHC(P)

CD4 Antibody (6A17): sc-70659

CD4 Antibody (6A17) is rated 4.5 out of 5 by 2.
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Datasheets

    • CD4 Antibody (6A17) is a mouse monoclonal IgG1 provided at 250 µl supernatant
    • raised against the extracellular domain of CD4 of human origin
    • recommended for detection of CD4 of human origin by WB, IP, IF and IHC(P)
    • See CD4 (MT310): sc-19641 for CD4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

I would like to use CD4 (6A17): sc-70659 for immunoprecipitation. Which protein agarose do you recommend using with this antibody?

Asked by: cjMara
Thank you for your question. We recommend using Protein A/G PLUS-Agarose: sc-2003 https://www.scbt.com/scbt/product/protein-a-g-plus-agarose
Answered by: Technical Support
Date published: 2017-04-12

Hello, I would like to know whether the anti-CD4 antibody (6A17) cat no sc-70659 needs a heat-mediated antigen retrieval for IHC-paraffin and if so what pH is recommended. Thank you

Asked by: Leonarda
Thank you for your inquiry. Yes, we do recommend antigen retrieval for IHC with paraffin-embedded sections. Heat treatment (recommended method): Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0; or with 50 mM glycine-HCl buffer (glycine: sc-29096), pH 3.5, with 0.01% (w/v) EDTA (EDTA: sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides. You can view our full protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Service
Date published: 2016-11-02
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Rated 4 out of 5 by from Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Date published: 2015-03-31
Rated 5 out of 5 by from Works well for WB with SUP Works well for WB with SUP-T1 and CCRF-CEM whole cell lysates. -SCBT QC
Date published: 2013-10-13
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