Anti-CD4 Antibody (18-46) recommended for detection of CD4 of human origin by IF and FCM

Anti-CD4 Antibody (18-46): sc-1176

Anti-CD4 Antibody (18-46) is rated 4.3 out of 5 by 6.
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Datasheets

    • CD4 Antibody (18-46) is a mouse monoclonal IgG2b κ, cited in 8 publications, provided at 200 µg/ml
    • recommended for detection of CD4 of human origin by IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • available conjugated to either APC or PerCP for IF, IHC(P) and FCM
    • See CD4 (MT310): sc-19641 for CD4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD4 Antibody (18-46). This work is in progress.
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Can CD4 (18-46): sc-1176 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD4 (18-46): sc-1176 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Does not work for IF I tried using this antibody to stain CD4+ T cells from PBMCs but saw no signal at a 1:50 dilution for 1h at RT.
Date published: 2016-11-05
Rated 5 out of 5 by from Produced exceptional FCM data for Hut78 cells Produced exceptional FCM data for Hut78 cells. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from Strong band in WB in human CD4 cells Strong band in WB in human CD4 cells. -SCBT Publication Review
Date published: 2015-04-16
Rated 5 out of 5 by from Good signal strength in WB in African monkey COS Good signal strength in WB in African monkey COS-7 cells. -SCBT Publication Review
Date published: 2015-03-17
Rated 5 out of 5 by from Good publishable data in WB in transfected Good publishable data in WB in transfected COS1 cells. -SCBT Publication Review
Date published: 2015-03-04
Rated 5 out of 5 by from Great FCM data of human peripheral blood Great FCM data of human peripheral blood leukocytes. -SCBT QC
Date published: 2013-03-26
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