CD33 Antibody (P67.6) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against CD33-transfected FMY9S5 cells of human origin
CD33 Antibody (P67.6) is recommended for detection of CD33 of human origin by IF and FCM
CD33 Antibody (P67.6) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD33 (P67.6): sc-19660.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
CD33 is a type I transmembrane glycoprotein that is found on granulocyte and macrophage precursors in the bone marrow, but is absent from pluripotent stem cells. CD33 is also expressed on monocytes in peripheral blood. It is used as a marker to distinguish myelogenous leukemia cells from lymphoid or erythroid leukemias. CD33 may function as a sialic acid-dependent cell adhesion molecule.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Thank you very much for your question. The sc-19660 is recommended for detection of CD33 of human origin by IF and FCM. If you have an interest in testing a free 10 µg sample of CD33 Antibody (P67.6) sc-19660 in western blot please contact Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27
What negative control should be used when using the conjugated version of CD33 (P67.6): sc-19660 monoclonal antibody in flow cytometry?
Asked by: jenni
Thank you for your inquiry. We recommend using the isotype specific normal mouse IgG conjugated to the same fluorophore. For example, if you are using sc-19660 PE, then you would want to use normal mouse IgG1 PE, sc-2866.
Rated 5 out of
Great FCM and IF dataStrong FCM and IF results; need to see IHC and western data
Date published: 2017-01-13
Rated 5 out of
Provides excellent FCM and IF dataThe CD33 Antibody (P67.6) proves to work great for flow cytometry applications, as well as, immunofluorescence applications showing strong staining localized to the membrane.
Date published: 2016-12-23
Rated 5 out of
Produced excellent immunofluorescence membraneProduced excellent immunofluorescence membrane staining in methanol-fixed THP-1 cells. -SCBT QC
Date published: 2015-02-11
Rated 5 out of
Great FCM data of THPGreat FCM data of THP-1 cells. -SCBT QC
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