CD33 Antibody (CD33-4D3)recommended for detection of CD33 of human origin by FCM

CD33 Antibody (CD33-4D3): sc-59020

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Datasheets

    • CD33 Antibody (CD33-4D3) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against CD33 of human origin
    • recommended for detection of CD33 of human origin by FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See CD33 (6C5/2): sc-53199 for CD33 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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If I want to use CD33 (CD33-4D3): sc-59020 for Flow Cytometry, what starting dilution do you recommend I use?

Asked by: Cweed
We recommend a starting dilution of 1µg of antibody per million cells. Here is a link to our recommended protocol: https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-03-24
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