CD32-A/C Antibody (B-3)recommended for detection of CD32-A and CD32-C of human origin by WB, IP, IF and ELISA

CD32-A/C Antibody (B-3): sc-373721

CD32-A/C Antibody (B-3) is rated 5.0 out of 5 by 3.
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Datasheets
    • CD32-A/C Antibody (B-3) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 293-317 at the C-terminus of CD32-A of human origin
    • CD32-A/C Antibody (B-3) is recommended for detection of CD32-A and CD32-C of human origin by WB, IP, IF and ELISA
    • CD32-A/C Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-373721 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD32-A/C (B-3): sc-373721.
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For the CD32A/C antibody, does one band correspond to A and one to C? Would it be possible to get a sample to test this antibody? I am wanting an antibody to just CD32A, but I see you discontinued it... Thanks, Lisa

Asked by: Lisa K
Thank you for your question. We have not characterized whether or not the two bands observed in the Western blot data on our website represent CD32A and C separately. CD32A has a predicted molecular weight of 35 kDa, while CD32C has a similar, but lower predicted molecular weight of 26-30 kDa. Yes, right now we are offering a free 10 µg sample of this monoclonal antibody, CD32-A/C (B-3): sc-373721. Please contact our Technical Service Department to request a free sample to test before you place your order. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-07-20

What application is the blocking peptide sc-373721 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Reliable Antibody, Great for WB Great for detecting CD32 of human origin via WB, very strong and clear signal with little background.
Date published: 2017-02-09
Rated 5 out of 5 by from Great Western Blot data of CD32 Great Western Blot data of CD32-A expression in non-transfected 293T, human CD32-A transfected 293T and U-937 whole cell lysates. -SCBT QC
Date published: 2015-05-02
Rated 5 out of 5 by from Good Western Blot data of CD32 Good Western Blot data of CD32-A/C expression in U-937 and AML-193 whole cell lysates. -SCBT QC
Date published: 2013-06-26
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