CD3-delta Antibody (F-1)recommended for detection of CD3-δ of human origin by WB, IP, IF and ELISA

CD3-δ Antibody (F-1): sc-137137

CD3-δ Antibody (F-1) is rated 4.7 out of 5 by 6.
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Datasheets

    • CD3-δ Antibody (F-1) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-171 representing full length CD3-δ of human origin
    • CD3-delta Antibody (F-1) is recommended for detection of CD3-δ of human origin by WB, IP, IF and ELISA
    • CD3-delta Antibody (F-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD3-δ (F-1): sc-137137.
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Does it recognize CD3-& from mouse by WB or only human?

Asked by: Maria Teresa
Thank you for your question. We have not yet confirmed reactivity with mouse CD3-delta using this antibody and so it is currently not recommended for that species.
Answered by: Technical Support
Date published: 2019-02-28

What is the recommended starting dilution for Western blot for sc-137137: CD3-δ (F-1) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from It is a great antibody The band of western bolt is very good, with 1: 500 dilution
Date published: 2017-10-18
Rated 5 out of 5 by from make great sense the result is essential for me , it gives me a new excited idea, I have dealt with my trouble. I used the CD3-delta antibody in 1:500 to test SY5Y cells by western blot.
Date published: 2017-03-27
Rated 5 out of 5 by from good probe CD3-delta antibaody is a satisfactory product,we will continue to support.
Date published: 2017-03-07
Rated 5 out of 5 by from Good antibody Good antibody with clear signal and no background, the antibody is very sensitive!
Date published: 2016-12-08
Rated 3 out of 5 by from it's not suited to IHC-P I tried IHC-P with this antibody, but there is no signal.
Date published: 2016-12-04
Rated 5 out of 5 by from Very robust signal produced against Jurkat Very robust signal produced against Jurkat, HuT 78 and CCRF-CEM whole cell lysates. -SCBT QC
Date published: 2014-05-05
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