Anti-CD26 Antibody (5E8) recommended for detection of CD26 of mouse and rat origin by WB, IP, IF and IHC(P)

Anti-CD26 Antibody (5E8): sc-52642

Anti-CD26 Antibody (5E8) is rated 4.4 out of 5 by 5.
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Datasheets

    • Anti-CD26 Antibody (5E8) is a mouse monoclonal IgG1 κ CD26 antibody, cited in 13 publications, provided at 200 µg/ml
    • raised against liver plasma membrane extracts of rat origin
    • Anti-CD26 Antibody (5E8) is recommended for detection of CD26 of mouse and rat origin by WB, IP, IF and IHC(P)
    • Anti-CD26 Antibody (5E8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD26 (5E8): sc-52642.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD26 Antibody (5E8) for WB and IHC(P) applications. These reagents are now offered in bundles with CD26 Antibody (5E8) (see ordering information below).
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I have use CD26 Antibody (5E8): sc-52642 on mouse kidney sections for IHC staining. Unfortunately, I didnot get any staining in proximal tubules of mice kidney. I checked all steps of protocol but still no staining

Asked by: b1978
Thank you for your question. CD26 Antibody (5E8): sc-52642 is a mouse monoclonal antibody that is suitable for detection of CD26 on mouse kidney formalin-fixed paraffin-embedded tissue sections. We have data on our website in rat kidney tissue showing apical membrane staining of cells in tubules. A link to our recommended protocol is provided below. Please contact our Technical Service Department if you would like help with troubleshooting the antibody. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-05-24

Can CD26 (5E8): sc-52642 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD26 (5E8): sc-52642 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24

这支抗体除了识别大鼠组织,在WB试验中,可以用于Human来源的细胞蛋白么? Does this antibody recognize protein from human cells?

Asked by: muyuzihegui
感谢您的提问。您可以联系我们的技术支持部门,我们会帮您进行序列比对分析,以确定该抗体能否用于您的样本。亚洲地区技术支持电话(+86) 021-60936351,邮箱[email protected]。 Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. Technical Services (Aisa): (+86) 021-60936351 or [email protected]
Answered by: Technical Support 12
Date published: 2017-01-06
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Rated 2 out of 5 by from Faint band... Tested in hepatic Huh 7.5 cells. Numerous faint bands, band at expected size extremely faint..
Date published: 2017-08-31
Rated 5 out of 5 by from 条带清晰 I have used this product one month ago.It is very clear in western bloting,very good!
Date published: 2017-01-17
Rated 5 out of 5 by from Positive result in WB in rat kidney tissue Positive result in WB in rat kidney tissue extract. -SCBT QC
Date published: 2015-05-30
Rated 5 out of 5 by from Band of interest observed in WB in rat PBL Band of interest observed in WB in rat PBL whole cell lysate. -SCBT QC
Date published: 2015-02-15
Rated 5 out of 5 by from Publishable data with rat liver sample Publishable data with rat liver sample - SCBT Publication Review
Date published: 2014-03-19
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