CD22 Antibody (MYG13)recommended for detection of CD22 of mouse, rat and human origin by WB, IP, IF and FCM

CD22 Antibody (MYG13): sc-7323

CD22 Antibody (MYG13) is rated 4.2 out of 5 by 5.
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Datasheets

    • CD22 Antibody (MYG13) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against Raji cells of human origin
    • CD22 Antibody (MYG13) is recommended for detection of CD22 of mouse, rat and human origin by WB, IP, IF and FCM
    • CD22 Antibody (MYG13) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD22 (MYG13): sc-7323.
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Can CD22 (MYG13): sc-7323 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD22 (MYG13): sc-7323 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Publishable data in flow using BJAB cells Publishable data in flow using BJAB cells for analysis. -SCBT Publication Review
Date published: 2015-07-17
Rated 4 out of 5 by from Robust FCM data of BJAB cells Robust FCM data of BJAB cells. -SCBT QC
Date published: 2015-04-08
Rated 4 out of 5 by from Nice publishable WB data on mouse spinal Nice publishable WB data on mouse spinal cord samples. -SCBT Publication Review
Date published: 2015-02-26
Rated 5 out of 5 by from Great antibody for flow cytometry Great antibody for flow cytometry, published data with peripheral blood mononuclear cells. -SCBT Publication Review
Date published: 2015-02-11
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