CD175s Antibody (3H1951)recommended for detection of CD175 on Glycophorin A and Glycophorin B in erythrocytes of human origin by IF

CD175s Antibody (3H1951): sc-70558

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Datasheets

    • CD175s Antibody (3H1951) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against CD175s red blood cells of human origin
    • recommended for detection of CD175 on Glycophorin A and Glycophorin B in erythrocytes of human origin by IF
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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What is the recommended fixation method for immunofluorescence staining with CD175s (3H1951): sc-70558 monoclonal antibody?

Asked by: Randy McDonald
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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