CD16 Antibody (3G8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
CD16 Antibody (3G8) is recommended for detection of CD16 of human origin by IF and FCM
Anti-CD16 Antibody (3G8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD16 (3G8): sc-19620.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
CD16, the low affinity Fc gamma receptor III for IgG (Fc γ RIII), exists both as a polypeptide-anchored form (Fc γ RIIIA or CD16-A) in human natural killer cells and macrophages and as a glycosylphosphatidylinositol-anchored form (FcγRIIIB or CD16-B) in neutrophils. CD16-A requires association of the γ subunit of FcεRI or the ζ subunit of the TCR-CD3 complex for cell surface expression. CD16-B is polymorphic; the two alleles are termed NA1 and NA2. CD16 is one of only four eukaryotic receptors known to exist natively in both the transmembrane (TM, CD16-A) and glycosylphosphatidylinositol (GPI, CD16-B) isoforms. Patients with paroxysmal nocturnal haemoglobinuria (PNH) have only about 10% of the normal levels of CD16 on their neutrophils, whereas the expression of FcRII is unaffected. Analysis of FcRIII expression in cells of PNH patients, known to be deficient in PI-linked proteins, suggests FcRIII is not PI-linked in monocytes.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
how can i get this monoclonal antibodies from your campany
Asked by: henok
Thank you for your question. For a limited time, we are offering a free 10 µg sample of this antibody. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-10-09
Can CD16 (3G8): sc-19620 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?
Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD16 (3G8): sc-19620 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn