CD16 Antibody (2Q1240)recommended for detection of CD16 of human origin by WB, IP, IF, IHC(P) and FCM

CD16 Antibody (2Q1240): sc-70548

CD16 Antibody (2Q1240) is rated 3.2 out of 5 by 5.
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Datasheets

    • CD16 Antibody (2Q1240) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against CD16
    • recommended for detection of CD16 of human origin by WB, IP, IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See CD16 (DJ130c): sc-20052 for CD16 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What is the recommended starting dilution for Western blot for sc-70548: CD16 (2Q1240) monoclonal antibody?

Asked by: TinTin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Works great IHC(P) and WB CD16 (2Q1240) gave a really strong signal in human PBL whole cell lysate for Western blot (WB).
Date published: 2017-02-03
Rated 3 out of 5 by from Publishable data in IHC(P) using human liver Publishable data in IHC(P) using human liver tissues -SCBT Publication Review
Date published: 2015-06-16
Rated 2 out of 5 by from Robust band seen in WB using human PBL whole Robust band seen in WB using human PBL whole cell lysate. -SCBT QC
Date published: 2015-05-25
Rated 3 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Date published: 2015-01-28
Rated 3 out of 5 by from Publishable data in IF using human NK cells Publishable data in IF using human NK cells -SCBT Publication Review
Date published: 2013-06-18
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