Anti-CCDC18 Antibody (G-5) recommended for detection of CCDC18 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-CCDC18 Antibody (G-5): sc-515316

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Datasheets

    • Anti-CCDC18 Antibody (G-5) is a mouse monoclonal IgG2b (kappa light chain) CCDC18 antibody provided at 200 µg/ml
    • raised against amino acids 801-933 mapping within an internal region of CCDC18 of human origin
    • Anti-CCDC18 Antibody (G-5) is recommended for detection of CCDC18 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-CCDC18 Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CCDC18 (G-5): sc-515316.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for CCDC18 Antibody (G-5) for WB applications. This reagent is now offered in a bundle with CCDC18 Antibody (G-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

What blocking solution should I use with CCDC18 (G-5): sc-515316 monoclonal antibody for Western blot?

Asked by: jenni
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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