catalase Antibody (H-9)recommended for detection of catalase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

catalase Antibody (H-9): sc-271803

catalase Antibody (H-9) is rated 4.7 out of 5 by 22.
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Datasheets

    • catalase Antibody (H-9) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 471-503 near the C-terminus of catalase of mouse origin
    • catalase Antibody (H-9) is recommended for detection of catalase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • catalase Antibody (H-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-271803 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of catalase (H-9): sc-271803.
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What is the difference between sc-271803 and sc-271803 P?

Asked by: jenni
Thank you for your question. sc-271803 P is our blocking peptide, meant as a negative control for use with the catalase (H-9): sc-271803 monoclonal antibody.
Answered by: Technical Support 4
Date published: 2018-02-24

What secondary is best to use with catalase (H-9): sc-271803?

Asked by: jenni
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with catalase (H-9):sc-271803, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-01-13
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Rated 4 out of 5 by from ottimo funzionamento Ho richiesto questo campione in omaggio insieme ad un ordine. L'ho preparato 1:100 in pbst 1% di marvel. Ottima risoluzione!
Date published: 2019-07-26
Rated 4 out of 5 by from Very good antibody This antibody has worked very well in my hands. I use a dilution of 1:1000. I am able to reuse the antibody as many as three times.
Date published: 2019-05-07
Rated 4 out of 5 by from Buon funzionamento l'anticorpo funziona bene in western blot su cellule umane di carcinoma pancreatico
Date published: 2018-12-05
Rated 5 out of 5 by from Works in IF This antibody works well in my immunostaining experiment with mouse samples.
Date published: 2018-06-05
Rated 5 out of 5 by from WB We used Catalase (H-9) antibody for WB in Mouse lung primary fibroblast, and it worked fine. Just remember use 1/250 to get good signal. We used 1/500 and it was a bit weak. We used anti-mouse IgG.
Date published: 2018-03-28
Rated 5 out of 5 by from excellent for WB i've been using this antibody (1/500) along with SOD1 (sc-101523: 1/500) and SOD2 (sc-133134: 1/10 000) to probe WB of rat thoracic aorta protein lysates. these 3 antibodies produce very distinct and clear/clean bands. catalase at 63 kd; SOD1 at 17 kD and SOD2 at 23 kD (green bands). beta-actin is in red at 42 kD.
Date published: 2018-02-14
Rated 5 out of 5 by from Excellent result Works really well with brain homogenate, using 1:500 dilution rate.
Date published: 2018-02-14
Rated 5 out of 5 by from works well The signal is very strong, and can be detected in MEFs. The diluted rate is 1:1000.
Date published: 2018-02-06
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