caspase-9 p35 Antibody (A-9)recommended for detection of p35 subunit and precursor of caspase-9 of mouse, rat and human origin by WB, IP, IF and ELISA

caspase-9 p35 Antibody (A-9): sc-133109

caspase-9 p35 Antibody (A-9) is rated 4.6 out of 5 by 10.
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Datasheets

    • caspase-9 p35 Antibody (A-9) is a mouse monoclonal IgG2a provided at 200 µg/ml
    • raised against amino acids 100-270 mapping within an internal region of caspase-9 p35 of human origin
    • caspase-9 p35 Antibody (A-9) is recommended for detection of p35 subunit and precursor of caspase-9 of mouse, rat and human origin by WB, IP, IF and ELISA
    • caspase-9 p35 Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-9 p35 (A-9): sc-133109.
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I am using caspase-9 p35 (A-9): sc-133109 monoclonal antibody for immunoprecipitation, how much antibody should I use?

Asked by: Professor Griffin
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-04-05
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Rated 3 out of 5 by from got a smear dilute 1:1000 in western blot, not clear full size band.
Date published: 2017-12-21
Rated 5 out of 5 by from Good band observed Used at 1:500 in HEK293 cells. The band is identical but signal is kind of weak might because I didn’t stimulate it too much.
Date published: 2017-12-12
Rated 5 out of 5 by from perfect It works good in U87 and Hela S3 cells at 1:1000 dilution
Date published: 2017-11-16
Rated 4 out of 5 by from good result good band in 40K in human peripheral blood mononuclear cells ,with 4 centigrade over 20 hour incubation of caspase-9 p35 antibody at 1:500.
Date published: 2017-10-12
Rated 5 out of 5 by from VERY GOOD ANTIBODY caspase-9 p35 Antibody (A-9) is as good as caspase-8 purchase from santa cruz.
Date published: 2017-08-30
Rated 5 out of 5 by from Excellent antibody I bought this antibody a month ago and it worked well in mice heart tissue extracts
Date published: 2017-08-30
Rated 5 out of 5 by from Great for WB The caspase-9 p35 Antibody (A-9): sc-133109 provides good Western blot data, showing clear bands with minimal background. This antibody has also been cited several times for further verification.
Date published: 2017-01-24
Rated 4 out of 5 by from Worked with transfected lysate by WB with Worked with transfected lysate by WB with some background -SCBT QC
Date published: 2015-02-19
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