caspase-9 p10 Antibody (F-7)recommended for detection of precursor and p10 subunit of caspase-9 of human origin by WB, IP, IF, IHC(P) and ELISA

caspase-9 p10 Antibody (F-7): sc-17784

caspase-9 p10 Antibody (F-7) is rated 4.9 out of 5 by 7.
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Datasheets

    • caspase-9 p10 Antibody (F-7) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 315-397 mapping within the C-terminus of caspase-9 p10 of human origin
    • caspase-9 p10 Antibody (F-7) is recommended for detection of precursor and p10 subunit of caspase-9 of human origin by WB, IP, IF, IHC(P) and ELISA
    • caspase-9 p10 Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-9 p10 (F-7): sc-17784.
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Can you recommend a positive control to use with caspase-9 p10 (F-7): sc-17784 monoclonal antibody for Western blot?

Asked by: jenni
We recommend Jurkat (sc-2204), K-562 (sc-2203), HCT-116 (sc-364175), Saos-2 (sc-2235), COLO 205 (sc-364177) or HEK 293 (sc-45136) whole cell lysates to use as positive controls as we have great Western blot data showing specific detection of caspase-9 p10 in these cell lines.
Answered by: Technical Support 15
Date published: 2017-01-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with caspase-9 p10 (F-7): sc-17784?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-12
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Rated 4 out of 5 by from GOOD Nice good antibody... Clear strong signal . .... .
Date published: 2017-12-21
Rated 5 out of 5 by from Good antibody for WB Used for detecting basal Caspase-9 level in 293T cells, dilute 1:1000 in western blot, clear full size band.
Date published: 2017-12-05
Rated 5 out of 5 by from Wow !! Superb product Just a great antibody for multiple applications; hard to compare !!
Date published: 2017-01-31
Rated 5 out of 5 by from Great caspase-9 antibody Great antibody with specificity to caspase-9 shown in siRNA knockdown studies. Great antibody for caspase-9 precursor and p10 detection.
Date published: 2017-01-27
Rated 5 out of 5 by from Used in Western with SGC Used in Western with SGC-7901, BGC-823 cells. - SCBT Publication Review
Date published: 2013-12-02
Rated 5 out of 5 by from Works with MDA Works with MDA-Panc-3, Panc-1, U2OS and SOAS2 cells in Western. - SCBT Publication Review
Date published: 2013-09-05
Rated 5 out of 5 by from Good results with RPMI Good results with RPMI-8226, OPM-2 , U-266 cells and T-cell leukaemia KE-37 cells. - SCBT Publication Review
Date published: 2008-01-15
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