caspase-9 Antibody (LAP 96-2-22)recommended for detection of caspase-9 of human origin by WB, IP and ELISA

caspase-9 Antibody (LAP 96-2-22): sc-56077

caspase-9 Antibody (LAP 96-2-22) is rated 5.0 out of 5 by 6.
  • y_2019, m_10, d_16, h_11
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.13
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_en_US, sid_56077, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 141ms
  • REVIEWS, PRODUCT
5
6
4
0
3
0
2
0
1
0

Datasheets

    • caspase-9 Antibody (LAP 96-2-22) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against full length caspase-9 of human origin
    • recommended for detection of caspase-9 of human origin by WB, IP and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See caspase-9 (96.1.23): sc-56076 for caspase-9 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
Submit a review for this product and receive 15 CruzCredits

I want to run a Western blot using this caspase-9 (LAP 96-2-22): sc-56077 monoclonal antibody. How much sample should I load on my gel?

Asked by: Professor Griffin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-05
  • y_2019, m_10, d_16, h_11CST
  • bvseo_bulk, prod_bvqa, vn_bulk_2.0.13
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_56077, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 86ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from good result Caspase-9 protein was used for rectal cancer detection, 1:800 dilution, antibody incubation at room temperature for 2 hours, we obtained the 45kda specific band.
Date published: 2017-09-16
Rated 5 out of 5 by from great Good WB antibody, and the effect was acceptable,nice
Date published: 2017-04-25
Rated 5 out of 5 by from Good antibody The caspase-9 Antibody (LAP 96-2-22): sc-56077 works well for Western blot applications showing minimal background noise. This antibody has also been cited several times for further verification.
Date published: 2017-01-19
Rated 5 out of 5 by from Great results with HL Great results with HL-60 cells. -SCBT Publication Review
Date published: 2013-09-25
Rated 5 out of 5 by from Works with porcine samples in WB Works with porcine samples in WB. -SCBT Publication Review
Date published: 2012-07-06
Rated 5 out of 5 by from Used with HeLa and SiHa in Western Used with HeLa and SiHa in Western. -SCBT Publication Review
Date published: 2011-06-25
  • y_2019, m_10, d_16, h_11
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.13
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_en_US, sid_56077, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 91ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.