caspase-9 Antibody (1-2)recommended for detection of the zymogen form of caspase-9 of human origin by WB and IP

caspase-9 Antibody (1-2): sc-56073

caspase-9 Antibody (1-2) is rated 4.8 out of 5 by 5.
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Datasheets

    • caspase-9 Antibody (1-2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against a fragment of caspase-9 of human origin
    • caspase-9 Antibody (1-2) is recommended for detection of the zymogen form of caspase-9 of human origin by WB and IP
    • caspase-9 Antibody (1-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-9 (1-2): sc-56073.
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using this single antibody can i see both procaspase9 and cleaved caspase 9 using westernblot (As u mentioned in one of your figures)?

Asked by: loga
Thank you for your question. This antibody is capable of detecting procaspase-9 and cleaved caspase-9 expression as long as it is present in your cells.
Answered by: Technical Support
Date published: 2019-07-13

What blocking solution should I use with caspase-9 (1-2): sc-56073 monoclonal antibody for Western blot?

Asked by: jerojero
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-13
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Rated 5 out of 5 by from Great Antibody for WB This antibody is great for WB and IP, has very strong and clear signal. Reliable monoclonal.
Date published: 2017-01-23
Rated 4 out of 5 by from This antibody worked well in WB This antibody worked well in WB.
Date published: 2015-06-23
Rated 5 out of 5 by from Good specificity and signal strength in CCRF Good specificity and signal strength in CCRF-CEM, HeLa and MOLT-4 whole cell lysates. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Good WB publishable data gathered with human Good WB publishable data gathered with human samples. -SCBT Publication Review
Date published: 2015-03-02
Rated 5 out of 5 by from Great WB antibody Great WB antibody! Published in Huh-7, Sk-hep1, Hep3B, and PLC5 cells. -SCBT Publication Review
Date published: 2015-01-08
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