caspase-8 p18 Antibody (E-8)recommended for detection of p18 subunit and precursor of caspase-8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

caspase-8 p18 Antibody (E-8): sc-166320

caspase-8 p18 Antibody (E-8) is rated 4.4 out of 5 by 5.
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Datasheets

    • caspase-8 p18 Antibody (E-8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 221-236 at the N-terminus of caspase-8 p18 of human origin
    • caspase-8 p18 Antibody (E-8) is recommended for detection of p18 subunit and precursor of caspase-8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • caspase-8 p18 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-166320 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-8 p18 (E-8): sc-166320.
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What application is the blocking peptide sc-166320 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from western blotting western blotting in human hepatocytes, the antibody can work well in 1:500, This antibody did work well.
Date published: 2017-06-07
Rated 5 out of 5 by from good IHC I used this antibody for IHC (dilution 1:100) and got very special staining in human duodenum tissue, very good.
Date published: 2017-01-15
Rated 5 out of 5 by from Robust staining in paraffin Robust staining in paraffin-embedded human duodenal tissue. -SCBT QC
Date published: 2015-01-23
Rated 3 out of 5 by from Works well for western blot with transfected Works well for western blot with transfected human samples. -SCBT QC
Date published: 2015-01-12
Rated 4 out of 5 by from Satisfactory cytoplasmic signal in HeLa cells Satisfactory cytoplasmic signal in HeLa cells. -SCBT QC
Date published: 2015-01-08
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