caspase-8 p18 Antibody (D-8)recommended for detection of p18 subunit and precursor of caspase-8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

caspase-8 p18 Antibody (D-8): sc-5263

caspase-8 p18 Antibody (D-8) is rated 4.8 out of 5 by 5.
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Datasheets

    • caspase-8 p18 Antibody (D-8) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against a recombinant protein corresponding to amino acids 217-350 mapping within the caspase-8 p18 subunit of human origin
    • caspase-8 p18 Antibody (D-8) is recommended for detection of p18 subunit and precursor of caspase-8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • caspase-8 p18 Antibody (D-8) is available conjugated to Alexa Fluor® 546 or Alexa Fluor® 594 for WB (RGB), IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-8 p18 (D-8): sc-5263.
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I need to use an antibody to detect the protein in samples of bovine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 9
Date published: 2017-01-11

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-11
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Rated 5 out of 5 by from Outstanding Very nice western data; also great IF and IHC staining
Date published: 2017-01-29
Rated 5 out of 5 by from Great antibody for IF The caspase-8 p18 Antibody (D-8): sc-5263 provides strong staining pattern for immunofluorescence localized to the cytoplasm and nucleus. This antibody has also been cited over 30 times for further verification.
Date published: 2017-01-12
Rated 5 out of 5 by from Good results with rat mammary glands in WB Good results with rat mammary glands in WB. - SCBT Publication Review
Date published: 2015-01-15
Rated 4 out of 5 by from Works well in Western with mouse ANA Works well in Western with mouse ANA-1 macrophages. - SCBT Publication Review
Date published: 2014-03-19
Rated 5 out of 5 by from Used with CHP Used with CHP-212 cells in Western. - SCBT Publication Review
Date published: 2013-09-01
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