caspase-8 Antibody (8CSP01)recommended for detection of caspase-8 of mouse, rat and human origin by WB and IP

caspase-8 Antibody (8CSP01): sc-81661

caspase-8 Antibody (8CSP01) is rated 4.0 out of 5 by 3.
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Datasheets

    • caspase-8 Antibody (8CSP01) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against full-length recombinant caspase-8 of human origin
    • recommended for detection of caspase-8 of mouse, rat and human origin by WB and IP
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See caspase-8 (8CSP03): sc-56070 for caspase-8 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What is the recommended starting dilution for Western blot for caspase-8 (8CSP01): sc-81661 monoclonal antibody?

Asked by: Germaine
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-81661.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Performs well in Western Performs well in Western, good signal strength with untreated and treated Jurkat whole cell lysates. -SCBT QC
Date published: 2015-04-02
Rated 5 out of 5 by from Performs well in Western Performs well in Western, good signal in MOLT-4 whole cell lysate. -SCBT QC
Date published: 2015-02-18
Rated 3 out of 5 by from Single strong band at correct mw with Jurkat Single strong band at correct mw with Jurkat but some background. -SCBT QC
Date published: 2015-02-13
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