caspase-8 Antibody (1.1.40)recommended for detection of caspase-8 of mouse, rat and human origin by WB and IP

caspase-8 Antibody (1.1.40): sc-81656

caspase-8 Antibody (1.1.40) is rated 4.0 out of 5 by 6.
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Datasheets

    • caspase-8 Antibody (1.1.40) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against full-length recombinant caspase-8 of human origin
    • caspase-8 Antibody (1.1.40) is recommended for detection of caspase-8 of mouse, rat and human origin by WB and IP
    • caspase-8 Antibody (1.1.40) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-8 (1.1.40): sc-81656.
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What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-20
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Rated 3 out of 5 by from good antibody This antibody was used in WB analysis in mouse tumor tissues, diluted 250 times in TBST 1x and milk 2%. I got bands in every sample, but faint.
Date published: 2018-05-11
Rated 2 out of 5 by from western blotting-okay for precursor band! This antibody was used for western blotting (1:200 dilution) of AC16 cell lysates. The precursor band was visible at the expected molecular weight but the cleaved caspase-8 band, though visible, was not at the predicted molecular weight.
Date published: 2018-04-27
Rated 5 out of 5 by from Great western Highly specific western; strong signal with low background
Date published: 2017-02-03
Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of caspase-8 precursor expression in Jurkat whole cell lysate.
Date published: 2017-01-12
Rated 4 out of 5 by from Performs well in Western Performs well in Western, good signal strength with untreated and treated Jurkat whole cell lysates. -SCBT QC
Date published: 2015-02-24
Rated 5 out of 5 by from Clean blot with single strong band in Jurkat Clean blot with single strong band in Jurkat whole cell lysate. -SCBT QC
Date published: 2015-01-06
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